By Debbie Emery - Breaking Celebrity News Reporter

Life has taken a serious turn for comedians Amy Poehler and Will Arnett, who separated on Thursday after nine years of marriage, a source has confirmed to Breaking Celebrity News.

The Parks And Recreation actress and the Up All Night actor married in August 2003, and have two sons together, Archie, 3, and Abel, 2.

Along with their individual achievements, Poehler and Arnett have appeared on-screen together in numerous successful projects, including Blades of Glory, Horton Hears a Who!, On Broadway, Spring Breakdown and Monsters vs. Aliens.

Saturday Night Live alum Amy, 40, also had a reoccurring role as the wife of 42-year-old Will's character, George Oscar "G.O.B." Bluth II, on the cult comedy Arrested Development.

Arnett was previously married to actress Penelope Ann Miller, who split from her second husband James Huggins last march, although they filed for divorce just a month after their marriage.

Both Poehler and Arnett are nominated for Primetime Emmy Awards and they will no doubt cross paths at the show on September 23.